Enzyme-linked immunosorbent assays or ELISA or EIA are blood tests that measure and detect antibodies. It can detect the presence of antibodies or proteins that have been produced in response to harmful substances known as antigens. This test is useful for diagnosing certain infections. ELISA can be used to diagnose HIV, Lyme Disease, Pernicious Anemia, and Varicella-Zoster.
It is used often as a screening tool prior to ordering more detailed tests. It is important to get accurate results. Here are some tips that will increase efficiency and accuracy when conducting ELISAs. High-quality ELISA kits are used to detect autoimmune-related antibodies in patients suffering from autoimmune disease.
The linked enzyme allows for the detection of antigens and antibodies in specific colors. It is also known as "enzyme linked immunosorbent assay" because ELISA tests are related to "immune", "enzyme". Some ELISA tests use signal amplifying techniques instead of enzymes. We like to refer to those that aren't related to immune or enzyme tests as ELISA.
In ELISA testing, ligand(s), conjugate with receptor(s), is accomplished with a color alter which has been detectable and measurable. We also use ligand receptors to substitute antigen or antibody and drop the description of the enzyme, expanding the definition. ELISA is a common "wet-laboratory" type of test. However, it uses a solid phase to detect presence of the substance.
The substance is typically in a liquid or wet specimen. ELISA tests can be performed with either 192 or 384 well plates. The ELISA test is widely used in medical diagnosis. It has also been used to detect plant disease pathogens in plant research. ELISA testing is used in many industries such as the food industry for quality control and assurance.